PneumaCult™は鼻粘膜上皮細胞の培養をサポートしますか?
鼻粘膜上皮細胞について直接の検証は行っておりませんが、PneumaCult™が同細胞タイプをサポートするとの報告があります。
以下の各文献をご参照ください。
PneumaCult™-Ex Plus Mediumによる増殖:
• Villamizar et al. (2019) Targeted Activation of Cystic Fibrosis Transmembrane Conductance Regulator. Mol Ther 27(10): 1737–48.
PneumaCult™-ALI Mediumによる分化:
・Müller L et al. (2013) Culturing of human nasal epithelial cells at the air liquid interface. J Vis Exp (80): 50646.
・Cao H et al. (2015) Testing gene therapy vectors in human primary nasal epithelial cultures. Mol Ther Methods Clin Dev 2: 15034.
・Schogler A et al. (2017) Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling. Respir Res 18(1):215.
・Garcia et al. (2019) Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures. Development 146(20): dev177428.
・Dobzanski et al. (2018) Nasal polyp fibroblasts modulate epithelial characteristics via Wnt signaling. Int Forum Allergy Rhinol 8(12):1412–20.
記事の評価: 参考になった